Easy and Rapid Purification of Highly Active Nisin
Joint Authors
Abts, André
Stindt, Jan
Mavaro, Antonino
Driessen, Arnold M. J.
Schmitt, Lutz
Smits, Sander H. J.
Bakkes, Patrick J.
Metzger, Sabine
Source
International Journal of Peptides
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nisin is an antimicrobial peptide produced and secreted by several L.
lactis strains and is specifically active against Gram-positive bacteria.
In previous studies, nisin was purified via cation exchange chromatography at low pH employing a single-step elution using 1 M NaCl.
Here, we describe an optimized purification protocol using a five-step NaCl elution to remove contaminants.
The obtained nisin is devoid of impurities and shows high bactericidal activity against the nisin-sensitive L.
lactis strain NZ9000.
Purified nisin exhibits an IC50 of ~3 nM, which is a tenfold improvement as compared to nisin obtained via the one-step elution procedure.
American Psychological Association (APA)
Abts, André& Mavaro, Antonino& Stindt, Jan& Bakkes, Patrick J.& Metzger, Sabine& Driessen, Arnold M. J.…[et al.]. 2011. Easy and Rapid Purification of Highly Active Nisin. International Journal of Peptides،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-451916
Modern Language Association (MLA)
Abts, André…[et al.]. Easy and Rapid Purification of Highly Active Nisin. International Journal of Peptides No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-451916
American Medical Association (AMA)
Abts, André& Mavaro, Antonino& Stindt, Jan& Bakkes, Patrick J.& Metzger, Sabine& Driessen, Arnold M. J.…[et al.]. Easy and Rapid Purification of Highly Active Nisin. International Journal of Peptides. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-451916
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451916
