Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells
المؤلفون المشاركون
Nicolete, Roberto
Zuliani, Juliana P.
Setúbal, Sulamita da S.
da Silva, Saulo Luís
Kayano, Anderson M.
Nomizo, Auro
Moura, Laura A.
Ribeiro, João G.
Stábeli, Rodrigo G.
Barros, Neuza B.
de Moura, Andrea A.
Calderon, Leonardo A.
Fuly, André Lopes
Soares, Andreimar Martins
Oliveira, George A.
Fernandes, Carla F. C.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-03
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Bothrops mattogrossensis snake is widely distributed throughout eastern South America and is responsible for snakebites in this region.
This paper reports the purification and biochemical characterization of three new phospholipases A2 (PLA2s), one of which is presumably an enzymatically active Asp49 and two are very likely enzymatically inactive Lys49 PLA2 homologues.
The purification was obtained after two chromatographic steps on ion exchange and reverse phase column.
The 2D SDS-PAGE analysis revealed that the proteins have pI values around 10, are each made of a single chain, and have molecular masses near 13 kDa, which was confirmed by MALDI-TOF mass spectrometry.
The N-terminal similarity analysis of the sequences showed that the proteins are highly homologous with other Lys49 and Asp49 PLA2s from Bothrops species.
The PLA2s isolated were named BmatTX-I (Lys49 PLA2-like), BmatTX-II (Lys49 PLA2-like), and BmatTX-III (Asp49 PLA2).
The PLA2s induced cytokine release from mouse neutrophils and showed cytotoxicity towards JURKAT (leukemia T) and SK-BR-3 (breast adenocarcinoma) cell lines and promastigote forms of Leishmania amazonensis.
The structural and functional elucidation of snake venoms components may contribute to a better understanding of the mechanism of action of these proteins during envenomation and their potential pharmacological and therapeutic applications.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
de Moura, Andrea A.& Kayano, Anderson M.& Oliveira, George A.& Setúbal, Sulamita da S.& Ribeiro, João G.& Barros, Neuza B.…[et al.]. 2014. Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-453596
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
de Moura, Andrea A.…[et al.]. Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-453596
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
de Moura, Andrea A.& Kayano, Anderson M.& Oliveira, George A.& Setúbal, Sulamita da S.& Ribeiro, João G.& Barros, Neuza B.…[et al.]. Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-453596
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-453596
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر