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Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells
Joint Authors
Nicolete, Roberto
Zuliani, Juliana P.
Setúbal, Sulamita da S.
da Silva, Saulo Luís
Kayano, Anderson M.
Nomizo, Auro
Moura, Laura A.
Ribeiro, João G.
Stábeli, Rodrigo G.
Barros, Neuza B.
de Moura, Andrea A.
Calderon, Leonardo A.
Fuly, André Lopes
Soares, Andreimar Martins
Oliveira, George A.
Fernandes, Carla F. C.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Bothrops mattogrossensis snake is widely distributed throughout eastern South America and is responsible for snakebites in this region.
This paper reports the purification and biochemical characterization of three new phospholipases A2 (PLA2s), one of which is presumably an enzymatically active Asp49 and two are very likely enzymatically inactive Lys49 PLA2 homologues.
The purification was obtained after two chromatographic steps on ion exchange and reverse phase column.
The 2D SDS-PAGE analysis revealed that the proteins have pI values around 10, are each made of a single chain, and have molecular masses near 13 kDa, which was confirmed by MALDI-TOF mass spectrometry.
The N-terminal similarity analysis of the sequences showed that the proteins are highly homologous with other Lys49 and Asp49 PLA2s from Bothrops species.
The PLA2s isolated were named BmatTX-I (Lys49 PLA2-like), BmatTX-II (Lys49 PLA2-like), and BmatTX-III (Asp49 PLA2).
The PLA2s induced cytokine release from mouse neutrophils and showed cytotoxicity towards JURKAT (leukemia T) and SK-BR-3 (breast adenocarcinoma) cell lines and promastigote forms of Leishmania amazonensis.
The structural and functional elucidation of snake venoms components may contribute to a better understanding of the mechanism of action of these proteins during envenomation and their potential pharmacological and therapeutic applications.
American Psychological Association (APA)
de Moura, Andrea A.& Kayano, Anderson M.& Oliveira, George A.& Setúbal, Sulamita da S.& Ribeiro, João G.& Barros, Neuza B.…[et al.]. 2014. Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-453596
Modern Language Association (MLA)
de Moura, Andrea A.…[et al.]. Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-453596
American Medical Association (AMA)
de Moura, Andrea A.& Kayano, Anderson M.& Oliveira, George A.& Setúbal, Sulamita da S.& Ribeiro, João G.& Barros, Neuza B.…[et al.]. Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-453596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453596