Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo
المؤلفون المشاركون
Rahman, Farhana
Shiba, Kiyotaka
Shinohara, Marie
Chowdhury, Mohammad Mahfuz
Fujita, Naoya
Danoy, Mathieu
Kaneda, Shohei
Fujii, Teruo
Sakai, Yasuyuki
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-11
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Organ-specific characteristic of endothelial cells (ECs) is crucial for specific adhesion of cancer cells to ECs, which is a key factor in the formation of organ-specific metastasis.
In this study, we developed a coculture of TMNK-1 (immortalized liver sinusoidal ECs) with 10T1/2 (resembling hepatic stellate cells) to augment organ-specific characteristic of TMNK-1 and investigated adhesion of two pancreatic cancer cells (MIA-PaCa-2 and BxPC-3) in the culture.
MIA-PaCa-2 and BxPC-3 adhesion in TMNK-1+10T1/2coating culture (TMNK-1 monolayer over 10T1/2 layer on collagen coated surface) were similar.
However, in TMNK-1+10T1/2gel (coculture on collagen gel surface), MIA-PaCa-2 adhesion was significantly higher than BxPC-3, which was congruent with the reported higher propensity of MIA-PaCa-2 than BxPC-3 to form liver metastasis in vivo.
Notably, as compared to BxPC-3, MIA-PaCa-2 adhesion was lower and similar in TMNK-1 only culture on the collagen coated and gel surfaces, respectively.
Investigation of the adhesion in the representative human umbilical vein ECs (HUVECs) cultures and upon blocking of surface molecules of ECs revealed that MIA-PaCa-2 adhesion was strongly dependent on the organ-specific upregulated characteristics of TMNK-1 in TMNK-1+10T1/2gel culture.
Therefore, the developed coculture would be a potential assay for screening novel drugs to inhibit the liver-microvasculature specific adhesion of cancer cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chowdhury, Mohammad Mahfuz& Danoy, Mathieu& Rahman, Farhana& Shinohara, Marie& Kaneda, Shohei& Shiba, Kiyotaka…[et al.]. 2014. Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-456642
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chowdhury, Mohammad Mahfuz…[et al.]. Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-456642
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chowdhury, Mohammad Mahfuz& Danoy, Mathieu& Rahman, Farhana& Shinohara, Marie& Kaneda, Shohei& Shiba, Kiyotaka…[et al.]. Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-456642
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-456642
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر