Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo
Joint Authors
Rahman, Farhana
Shiba, Kiyotaka
Shinohara, Marie
Chowdhury, Mohammad Mahfuz
Fujita, Naoya
Danoy, Mathieu
Kaneda, Shohei
Fujii, Teruo
Sakai, Yasuyuki
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Organ-specific characteristic of endothelial cells (ECs) is crucial for specific adhesion of cancer cells to ECs, which is a key factor in the formation of organ-specific metastasis.
In this study, we developed a coculture of TMNK-1 (immortalized liver sinusoidal ECs) with 10T1/2 (resembling hepatic stellate cells) to augment organ-specific characteristic of TMNK-1 and investigated adhesion of two pancreatic cancer cells (MIA-PaCa-2 and BxPC-3) in the culture.
MIA-PaCa-2 and BxPC-3 adhesion in TMNK-1+10T1/2coating culture (TMNK-1 monolayer over 10T1/2 layer on collagen coated surface) were similar.
However, in TMNK-1+10T1/2gel (coculture on collagen gel surface), MIA-PaCa-2 adhesion was significantly higher than BxPC-3, which was congruent with the reported higher propensity of MIA-PaCa-2 than BxPC-3 to form liver metastasis in vivo.
Notably, as compared to BxPC-3, MIA-PaCa-2 adhesion was lower and similar in TMNK-1 only culture on the collagen coated and gel surfaces, respectively.
Investigation of the adhesion in the representative human umbilical vein ECs (HUVECs) cultures and upon blocking of surface molecules of ECs revealed that MIA-PaCa-2 adhesion was strongly dependent on the organ-specific upregulated characteristics of TMNK-1 in TMNK-1+10T1/2gel culture.
Therefore, the developed coculture would be a potential assay for screening novel drugs to inhibit the liver-microvasculature specific adhesion of cancer cells.
American Psychological Association (APA)
Chowdhury, Mohammad Mahfuz& Danoy, Mathieu& Rahman, Farhana& Shinohara, Marie& Kaneda, Shohei& Shiba, Kiyotaka…[et al.]. 2014. Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-456642
Modern Language Association (MLA)
Chowdhury, Mohammad Mahfuz…[et al.]. Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-456642
American Medical Association (AMA)
Chowdhury, Mohammad Mahfuz& Danoy, Mathieu& Rahman, Farhana& Shinohara, Marie& Kaneda, Shohei& Shiba, Kiyotaka…[et al.]. Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-456642
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456642