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Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site
المؤلفون المشاركون
Maeda, Eiichi
Arizono, Naoki
Goto, Shuji
Takaoka, Yutaka
Hirota, Yushi
Ishizuka, Hiroshi
Ohta, Mika
Sugano, Aki
Nishio, Hisahide
Sato, Nobuko
Funakoshi, Kotaro
Takaoka, Nobuo
Yusoff, Surini
Miura, Kenji
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-02-17
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
We have been investigating the molecular efficacy of electroacupuncture (EA), which is one type of acupuncture therapy.
In our previous molecular biological study of acupuncture, we found an EA-induced gene, named acupuncture-induced 1-L (Aig1l), in mouse skeletal muscle.
The aims of this study consisted of identification of the full-length cDNA sequence of Aig1l including the transcriptional start site, determination of the tissue distribution of Aig1l and analysis of the effect of EA on Aig1l gene expression.
We determined the complete cDNA sequence including the transcriptional start site via cDNA cloning with the cap site hunting method.
We then analyzed the tissue distribution of Aig1l by means of northern blot analysis and real-time quantitative polymerase chain reaction.
We used the semiquantitative reverse transcriptase-polymerase chain reaction to examine the effect of EA on Aig1l gene expression.
Our results showed that the complete cDNA sequence of Aig1l was 6073 bp long, and the putative protein consisted of 962 amino acids.
All seven tissues that we analyzed expressed the Aig1l gene.
In skeletal muscle, EA induced expression of the Aig1l gene, with high expression observed after 3 hours of EA.
Our findings thus suggest that the Aig1l gene may play a key role in the molecular mechanisms of EA efficacy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ohta, Mika& Sugano, Aki& Goto, Shuji& Yusoff, Surini& Hirota, Yushi& Funakoshi, Kotaro…[et al.]. 2011. Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457283
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ohta, Mika…[et al.]. Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-457283
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ohta, Mika& Sugano, Aki& Goto, Shuji& Yusoff, Surini& Hirota, Yushi& Funakoshi, Kotaro…[et al.]. Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457283
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-457283
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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