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Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site
Joint Authors
Maeda, Eiichi
Arizono, Naoki
Goto, Shuji
Takaoka, Yutaka
Hirota, Yushi
Ishizuka, Hiroshi
Ohta, Mika
Sugano, Aki
Nishio, Hisahide
Sato, Nobuko
Funakoshi, Kotaro
Takaoka, Nobuo
Yusoff, Surini
Miura, Kenji
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We have been investigating the molecular efficacy of electroacupuncture (EA), which is one type of acupuncture therapy.
In our previous molecular biological study of acupuncture, we found an EA-induced gene, named acupuncture-induced 1-L (Aig1l), in mouse skeletal muscle.
The aims of this study consisted of identification of the full-length cDNA sequence of Aig1l including the transcriptional start site, determination of the tissue distribution of Aig1l and analysis of the effect of EA on Aig1l gene expression.
We determined the complete cDNA sequence including the transcriptional start site via cDNA cloning with the cap site hunting method.
We then analyzed the tissue distribution of Aig1l by means of northern blot analysis and real-time quantitative polymerase chain reaction.
We used the semiquantitative reverse transcriptase-polymerase chain reaction to examine the effect of EA on Aig1l gene expression.
Our results showed that the complete cDNA sequence of Aig1l was 6073 bp long, and the putative protein consisted of 962 amino acids.
All seven tissues that we analyzed expressed the Aig1l gene.
In skeletal muscle, EA induced expression of the Aig1l gene, with high expression observed after 3 hours of EA.
Our findings thus suggest that the Aig1l gene may play a key role in the molecular mechanisms of EA efficacy.
American Psychological Association (APA)
Ohta, Mika& Sugano, Aki& Goto, Shuji& Yusoff, Surini& Hirota, Yushi& Funakoshi, Kotaro…[et al.]. 2011. Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457283
Modern Language Association (MLA)
Ohta, Mika…[et al.]. Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-457283
American Medical Association (AMA)
Ohta, Mika& Sugano, Aki& Goto, Shuji& Yusoff, Surini& Hirota, Yushi& Funakoshi, Kotaro…[et al.]. Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457283