Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples
المؤلفون المشاركون
Kronfeldt, Heinz-Detlef
Sowoidnich, Kay
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-08-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Shifted excitation Raman difference spectroscopy (SERDS) was applied for an effective fluorescence removal in the Raman spectra of meat, fat, connective tissue, and bone from pork and beef.
As excitation light sources, microsystem diode lasers emitting at 783 nm, 671 nm, and 488 nm each incorporating two slightly shifted excitation wavelengths with a spectral difference of about 10 cm−1 necessary for SERDS operation were used.
The moderate fluorescence interference for 783 nm excitation as well as the increased background level at 671 nm was efficiently rejected using SERDS resulting in a straight horizontal baseline.
This allows for identification of all characteristic Raman signals including weak bands which are clearly visible and overlapping signals that are resolved in the SERDS spectra.
At 488 nm excitation, the spectra contain an overwhelming fluorescence interference masking nearly all Raman signals of the probed tissue samples.
However, the essentially background-free SERDS spectra enable determining the majority of characteristic Raman bands of the samples under investigation.
Furthermore, 488 nm excitation reveals prominent carotenoid signals enhanced due to resonance Raman scattering which are present in the beef samples but absent in pork tissue enabling a rapid meat species differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sowoidnich, Kay& Kronfeldt, Heinz-Detlef. 2012. Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples. ISRN Spectroscopy،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-457859
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sowoidnich, Kay& Kronfeldt, Heinz-Detlef. Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples. ISRN Spectroscopy No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-457859
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sowoidnich, Kay& Kronfeldt, Heinz-Detlef. Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples. ISRN Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-457859
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-457859
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر