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Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples
Joint Authors
Kronfeldt, Heinz-Detlef
Sowoidnich, Kay
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Shifted excitation Raman difference spectroscopy (SERDS) was applied for an effective fluorescence removal in the Raman spectra of meat, fat, connective tissue, and bone from pork and beef.
As excitation light sources, microsystem diode lasers emitting at 783 nm, 671 nm, and 488 nm each incorporating two slightly shifted excitation wavelengths with a spectral difference of about 10 cm−1 necessary for SERDS operation were used.
The moderate fluorescence interference for 783 nm excitation as well as the increased background level at 671 nm was efficiently rejected using SERDS resulting in a straight horizontal baseline.
This allows for identification of all characteristic Raman signals including weak bands which are clearly visible and overlapping signals that are resolved in the SERDS spectra.
At 488 nm excitation, the spectra contain an overwhelming fluorescence interference masking nearly all Raman signals of the probed tissue samples.
However, the essentially background-free SERDS spectra enable determining the majority of characteristic Raman bands of the samples under investigation.
Furthermore, 488 nm excitation reveals prominent carotenoid signals enhanced due to resonance Raman scattering which are present in the beef samples but absent in pork tissue enabling a rapid meat species differentiation.
American Psychological Association (APA)
Sowoidnich, Kay& Kronfeldt, Heinz-Detlef. 2012. Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples. ISRN Spectroscopy،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-457859
Modern Language Association (MLA)
Sowoidnich, Kay& Kronfeldt, Heinz-Detlef. Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples. ISRN Spectroscopy No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-457859
American Medical Association (AMA)
Sowoidnich, Kay& Kronfeldt, Heinz-Detlef. Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples. ISRN Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-457859
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457859