Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori
المؤلفون المشاركون
Tanih, Nicoline F.
Ndip, Roland N.
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-24
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Rapid diagnosis and treatment of Helicobacter pylori (H.
pylori) presents a challenge.
We aimed at investigating the presence of H.
pylori, susceptibility profile, and associated mutations in an effort to validate the effectiveness of GenoType HelicoDR assay in H.
pylori typing in our environment.
Two hundred and fifty-four biopsy specimens were cultured and DNA extracted from seventy-eight positive cultures using the Qiagen DNA extraction kit.
The GenoType Helico DR which employs reverse hybridisation was used to confirm the presence of H.
pylori, determination of its susceptibility to antimicrobials, and detection of mutations conferring resistance to clarithromycin and fluoroquinolones.
The organism was isolated from 168/254 (66.1 %) of the specimens by culture.
Of the 78 strains used for further investigation, 12/78 (15.38%) were resistant to clarithromycin while 66/78 (84.61%) were susceptible.
For fluoroquinolone, 70/78 (89.74%) strains were susceptible while 8 (10.26%) were resistant.
Mutations were observed in 17 strains with A2147G being the most prevalent; A2146C and D91N were the least.
The reverse hybridisation assay is an easy and fast technique in confirming the presence of H.
pylori, its antimicrobial profile, and associated mutations.
Analysis regarding the suitability of this assay for H.
pylori typing is warranted in other regions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tanih, Nicoline F.& Ndip, Roland N.. 2013. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterology Research and Practice،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-458190
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tanih, Nicoline F.& Ndip, Roland N.. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterology Research and Practice No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-458190
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tanih, Nicoline F.& Ndip, Roland N.. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterology Research and Practice. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-458190
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-458190
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر