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Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori
Joint Authors
Tanih, Nicoline F.
Ndip, Roland N.
Source
Gastroenterology Research and Practice
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Rapid diagnosis and treatment of Helicobacter pylori (H.
pylori) presents a challenge.
We aimed at investigating the presence of H.
pylori, susceptibility profile, and associated mutations in an effort to validate the effectiveness of GenoType HelicoDR assay in H.
pylori typing in our environment.
Two hundred and fifty-four biopsy specimens were cultured and DNA extracted from seventy-eight positive cultures using the Qiagen DNA extraction kit.
The GenoType Helico DR which employs reverse hybridisation was used to confirm the presence of H.
pylori, determination of its susceptibility to antimicrobials, and detection of mutations conferring resistance to clarithromycin and fluoroquinolones.
The organism was isolated from 168/254 (66.1 %) of the specimens by culture.
Of the 78 strains used for further investigation, 12/78 (15.38%) were resistant to clarithromycin while 66/78 (84.61%) were susceptible.
For fluoroquinolone, 70/78 (89.74%) strains were susceptible while 8 (10.26%) were resistant.
Mutations were observed in 17 strains with A2147G being the most prevalent; A2146C and D91N were the least.
The reverse hybridisation assay is an easy and fast technique in confirming the presence of H.
pylori, its antimicrobial profile, and associated mutations.
Analysis regarding the suitability of this assay for H.
pylori typing is warranted in other regions.
American Psychological Association (APA)
Tanih, Nicoline F.& Ndip, Roland N.. 2013. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterology Research and Practice،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-458190
Modern Language Association (MLA)
Tanih, Nicoline F.& Ndip, Roland N.. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterology Research and Practice No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-458190
American Medical Association (AMA)
Tanih, Nicoline F.& Ndip, Roland N.. Molecular Detection of Antibiotic Resistance in South African Isolates of Helicobacter pylori. Gastroenterology Research and Practice. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-458190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458190