Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract
المؤلفون المشاركون
Tanaka, Hiroyuki
Li, Xiao Wei
Aboagye, Frederick Asare
Fuji, Shunsuke
Putalun, Waraporn
Shoyama, Yukihiro
Morinaga, Osamu
Tung, Nguyen Huu
Uto, Takuhiro
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-02-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry was used for the confirmation of hapten number in synthesized antigen.
As application of MAb, the MAbs against ginsenosides and glycyrrhizin have been prepared resulting in the development of two new techniques that we named the eastern blotting method and the knockout extract preparation.
In eastern blotting technique, glycosides like ginsenosides and glycyrrhizin separated by silica gel TLC were blotted to PVDF membrane that was treated with a NaIO4 solution followed by BSA resulted in glycoside-BSA conjugate on a PVDF membrane.
The blotted spots were stained by MAb.
Double staining of eastern blotting for ginsenosides using antiginsenoside Rb1 and Rg1 MAbs promoted complete identification of ginsenosides in Panax species.
The immunoaffinity concentration of glycyrrhizin was determined by immunoaffinity column conjugated with antiglycyrrhizin MAb resulting in the glycyrrhizin-knockout extract, which was determined by the synergic effect with glycyrrhizin on NO production using the cell line.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tanaka, Hiroyuki& Morinaga, Osamu& Uto, Takuhiro& Fuji, Shunsuke& Aboagye, Frederick Asare& Tung, Nguyen Huu…[et al.]. 2012. Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract. International Journal of Analytical Chemistry،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-458266
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tanaka, Hiroyuki…[et al.]. Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract. International Journal of Analytical Chemistry No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-458266
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tanaka, Hiroyuki& Morinaga, Osamu& Uto, Takuhiro& Fuji, Shunsuke& Aboagye, Frederick Asare& Tung, Nguyen Huu…[et al.]. Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract. International Journal of Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-458266
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-458266
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر