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Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract
Joint Authors
Tanaka, Hiroyuki
Li, Xiao Wei
Aboagye, Frederick Asare
Fuji, Shunsuke
Putalun, Waraporn
Shoyama, Yukihiro
Morinaga, Osamu
Tung, Nguyen Huu
Uto, Takuhiro
Source
International Journal of Analytical Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry was used for the confirmation of hapten number in synthesized antigen.
As application of MAb, the MAbs against ginsenosides and glycyrrhizin have been prepared resulting in the development of two new techniques that we named the eastern blotting method and the knockout extract preparation.
In eastern blotting technique, glycosides like ginsenosides and glycyrrhizin separated by silica gel TLC were blotted to PVDF membrane that was treated with a NaIO4 solution followed by BSA resulted in glycoside-BSA conjugate on a PVDF membrane.
The blotted spots were stained by MAb.
Double staining of eastern blotting for ginsenosides using antiginsenoside Rb1 and Rg1 MAbs promoted complete identification of ginsenosides in Panax species.
The immunoaffinity concentration of glycyrrhizin was determined by immunoaffinity column conjugated with antiglycyrrhizin MAb resulting in the glycyrrhizin-knockout extract, which was determined by the synergic effect with glycyrrhizin on NO production using the cell line.
American Psychological Association (APA)
Tanaka, Hiroyuki& Morinaga, Osamu& Uto, Takuhiro& Fuji, Shunsuke& Aboagye, Frederick Asare& Tung, Nguyen Huu…[et al.]. 2012. Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract. International Journal of Analytical Chemistry،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-458266
Modern Language Association (MLA)
Tanaka, Hiroyuki…[et al.]. Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract. International Journal of Analytical Chemistry No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-458266
American Medical Association (AMA)
Tanaka, Hiroyuki& Morinaga, Osamu& Uto, Takuhiro& Fuji, Shunsuke& Aboagye, Frederick Asare& Tung, Nguyen Huu…[et al.]. Application of Monoclonal Antibodies against Bioactive Natural Products : Eastern Blotting and Preparation of Knockout Extract. International Journal of Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-458266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-458266