![](/images/graphics-bg.png)
Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia
المؤلفون المشاركون
Erdfelder, Felix
Uhrmacher, Sabrina
Kreuzer, Karl-Anton
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-09-20
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
New therapeutic strategies developed recently for chronic lymphocytic leukemia (CLL) have led to remarkable treatment response rates and complete hematological remissions.
This means highly sensitive and specific techniques are increasingly needed to evaluate minimal residual disease (MRD) in CLL patients.
Quantitative MRD levels can be used as prognostic markers, where total MRD eradication is associated with prolonged survival.
Nowadays, PCR and flow cytometry techniques used to detect MRD in CLL patients can generate reliable and quantitative results with the highest sensitivity.
MRD Flow is based on four-color flow cytometry using specific antibody combinations.
For allele specific oligonucleotide real-time quantification (ASO RQ) PCR individual primers are designed to detect a specific immunoglobulin heavy chain (IgH) rearrangement in each patient clone.
Five comprehensive studies investigated and compared the sensitivity and specificity of both methods.
Groups of patients receiving different therapies were analyzed at different time points to generate quantitative MRD levels and MRD kinetics.
All studies confirmed that both methods generate equivalent results with regard to sensitivity and MRD quantification, although each method has advantages and disadvantages in the daily routine of a standard hematological laboratory.
Here, we review these investigations and compare their results in the light of modern therapies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Uhrmacher, Sabrina& Erdfelder, Felix& Kreuzer, Karl-Anton. 2010. Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia. Advances in Hematology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-459290
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Uhrmacher, Sabrina…[et al.]. Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia. Advances in Hematology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-459290
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Uhrmacher, Sabrina& Erdfelder, Felix& Kreuzer, Karl-Anton. Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia. Advances in Hematology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-459290
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-459290
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)