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Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia
Joint Authors
Erdfelder, Felix
Uhrmacher, Sabrina
Kreuzer, Karl-Anton
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
New therapeutic strategies developed recently for chronic lymphocytic leukemia (CLL) have led to remarkable treatment response rates and complete hematological remissions.
This means highly sensitive and specific techniques are increasingly needed to evaluate minimal residual disease (MRD) in CLL patients.
Quantitative MRD levels can be used as prognostic markers, where total MRD eradication is associated with prolonged survival.
Nowadays, PCR and flow cytometry techniques used to detect MRD in CLL patients can generate reliable and quantitative results with the highest sensitivity.
MRD Flow is based on four-color flow cytometry using specific antibody combinations.
For allele specific oligonucleotide real-time quantification (ASO RQ) PCR individual primers are designed to detect a specific immunoglobulin heavy chain (IgH) rearrangement in each patient clone.
Five comprehensive studies investigated and compared the sensitivity and specificity of both methods.
Groups of patients receiving different therapies were analyzed at different time points to generate quantitative MRD levels and MRD kinetics.
All studies confirmed that both methods generate equivalent results with regard to sensitivity and MRD quantification, although each method has advantages and disadvantages in the daily routine of a standard hematological laboratory.
Here, we review these investigations and compare their results in the light of modern therapies.
American Psychological Association (APA)
Uhrmacher, Sabrina& Erdfelder, Felix& Kreuzer, Karl-Anton. 2010. Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia. Advances in Hematology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-459290
Modern Language Association (MLA)
Uhrmacher, Sabrina…[et al.]. Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia. Advances in Hematology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-459290
American Medical Association (AMA)
Uhrmacher, Sabrina& Erdfelder, Felix& Kreuzer, Karl-Anton. Flow Cytometry and Polymerase Chain Reaction-Based Analyses of Minimal Residual Disease in Chronic Lymphocytic Leukemia. Advances in Hematology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-459290
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459290