Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo
المؤلفون المشاركون
Longo-Mbenza, Benjamin
Eric, Kendjo
Jean, Sala
Madone, Mandina
Marc, Thellier
AC, Guillo-Olczyk
Fabien, Kintoki
Jean, Menotti
Josué, Zanga
Wumba, Roger
المصدر
Journal of Parasitology Research
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-07-01
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Objective.
To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients.
Methods.
This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012.
Detection of microsporidia including E.
bieneusi and E.
intestinalis was performed in 242 HIV-infected patients.
Typing was based on DNA polymorphism of the ribosomal DNA ITS region of E.
bieneusi.
PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages.
The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard.
Results.
Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of E.
bieneusi was 7.9% (n=19) among the 19 E.
bieneusi, one was coinfected with E.
intestinalis.
In 19 E.
bieneusi persons using PCR-RFLP method, 5 type I strains of E.
bieneusi (26.3%) and 5 type IV strains of E.
bieneusi (26.3%) were identified.
The sensitivity of IFI-AcM was poor as estimated 42.1%.
Conclusion.
Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wumba, Roger& Jean, Menotti& Longo-Mbenza, Benjamin& Madone, Mandina& Fabien, Kintoki& Josué, Zanga…[et al.]. 2012. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-459692
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wumba, Roger…[et al.]. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-459692
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wumba, Roger& Jean, Menotti& Longo-Mbenza, Benjamin& Madone, Mandina& Fabien, Kintoki& Josué, Zanga…[et al.]. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-459692
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-459692
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر