![](/images/graphics-bg.png)
Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo
المؤلفون المشاركون
Longo-Mbenza, Benjamin
Eric, Kendjo
Jean, Sala
Madone, Mandina
Marc, Thellier
AC, Guillo-Olczyk
Fabien, Kintoki
Jean, Menotti
Josué, Zanga
Wumba, Roger
المصدر
Journal of Parasitology Research
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-07-01
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Objective.
To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients.
Methods.
This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012.
Detection of microsporidia including E.
bieneusi and E.
intestinalis was performed in 242 HIV-infected patients.
Typing was based on DNA polymorphism of the ribosomal DNA ITS region of E.
bieneusi.
PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages.
The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard.
Results.
Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of E.
bieneusi was 7.9% (n=19) among the 19 E.
bieneusi, one was coinfected with E.
intestinalis.
In 19 E.
bieneusi persons using PCR-RFLP method, 5 type I strains of E.
bieneusi (26.3%) and 5 type IV strains of E.
bieneusi (26.3%) were identified.
The sensitivity of IFI-AcM was poor as estimated 42.1%.
Conclusion.
Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wumba, Roger& Jean, Menotti& Longo-Mbenza, Benjamin& Madone, Mandina& Fabien, Kintoki& Josué, Zanga…[et al.]. 2012. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-459692
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wumba, Roger…[et al.]. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-459692
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wumba, Roger& Jean, Menotti& Longo-Mbenza, Benjamin& Madone, Mandina& Fabien, Kintoki& Josué, Zanga…[et al.]. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-459692
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-459692
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)