Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo
Joint Authors
Longo-Mbenza, Benjamin
Eric, Kendjo
Jean, Sala
Madone, Mandina
Marc, Thellier
AC, Guillo-Olczyk
Fabien, Kintoki
Jean, Menotti
Josué, Zanga
Wumba, Roger
Source
Journal of Parasitology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients.
Methods.
This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012.
Detection of microsporidia including E.
bieneusi and E.
intestinalis was performed in 242 HIV-infected patients.
Typing was based on DNA polymorphism of the ribosomal DNA ITS region of E.
bieneusi.
PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages.
The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard.
Results.
Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of E.
bieneusi was 7.9% (n=19) among the 19 E.
bieneusi, one was coinfected with E.
intestinalis.
In 19 E.
bieneusi persons using PCR-RFLP method, 5 type I strains of E.
bieneusi (26.3%) and 5 type IV strains of E.
bieneusi (26.3%) were identified.
The sensitivity of IFI-AcM was poor as estimated 42.1%.
Conclusion.
Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.
American Psychological Association (APA)
Wumba, Roger& Jean, Menotti& Longo-Mbenza, Benjamin& Madone, Mandina& Fabien, Kintoki& Josué, Zanga…[et al.]. 2012. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-459692
Modern Language Association (MLA)
Wumba, Roger…[et al.]. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-459692
American Medical Association (AMA)
Wumba, Roger& Jean, Menotti& Longo-Mbenza, Benjamin& Madone, Mandina& Fabien, Kintoki& Josué, Zanga…[et al.]. Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo. Journal of Parasitology Research. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-459692
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-459692