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Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues
المؤلفون المشاركون
Gan, Wei
Cai, Jian-Ping
Hu, Guo-Xin
Chen, Lian-Guo
Sekiguchi, Mutsuo
Shi, Fei
Hayakawa, Hiroshi
Nie, Ben
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats.
For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS method to determine the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn) in DNA and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) in RNA.
An age-dependent increase in oxidative DNA and RNA damage was observed in the various organs examined, including the brain, liver, kidneys, and testes.
Similar increases in the 8-oxo-dGsn and 8-oxo-Gsn contents were observed in three parts of the brain, the hippocampus, cerebral cortex, and cerebellum, among which, the values for the hippocampus were always the highest.
When the oxidized guanosine metabolites were quantified with urine, a similar age-dependent increase was observed for both 8-oxo-dGsn and 8-oxo-Gsn.
However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation.
Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nie, Ben& Gan, Wei& Shi, Fei& Hu, Guo-Xin& Chen, Lian-Guo& Hayakawa, Hiroshi…[et al.]. 2013. Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-461767
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nie, Ben…[et al.]. Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-461767
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nie, Ben& Gan, Wei& Shi, Fei& Hu, Guo-Xin& Chen, Lian-Guo& Hayakawa, Hiroshi…[et al.]. Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-461767
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-461767
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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