Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues
Joint Authors
Gan, Wei
Cai, Jian-Ping
Hu, Guo-Xin
Chen, Lian-Guo
Sekiguchi, Mutsuo
Shi, Fei
Hayakawa, Hiroshi
Nie, Ben
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats.
For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS method to determine the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn) in DNA and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) in RNA.
An age-dependent increase in oxidative DNA and RNA damage was observed in the various organs examined, including the brain, liver, kidneys, and testes.
Similar increases in the 8-oxo-dGsn and 8-oxo-Gsn contents were observed in three parts of the brain, the hippocampus, cerebral cortex, and cerebellum, among which, the values for the hippocampus were always the highest.
When the oxidized guanosine metabolites were quantified with urine, a similar age-dependent increase was observed for both 8-oxo-dGsn and 8-oxo-Gsn.
However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation.
Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging.
American Psychological Association (APA)
Nie, Ben& Gan, Wei& Shi, Fei& Hu, Guo-Xin& Chen, Lian-Guo& Hayakawa, Hiroshi…[et al.]. 2013. Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-461767
Modern Language Association (MLA)
Nie, Ben…[et al.]. Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-461767
American Medical Association (AMA)
Nie, Ben& Gan, Wei& Shi, Fei& Hu, Guo-Xin& Chen, Lian-Guo& Hayakawa, Hiroshi…[et al.]. Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-461767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-461767