Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors

الملخص EN

IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity.

A number of SNPs that influence IL-18 production are found in the gene promoter region.

We investigated the association of SNPs in the IL-18 promoter at −607 and −137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia.

The genetic distribution of these SNPs in the promoter site was established by PCR.

IL-18 protein production was determined by ELISA.

Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production.

In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type.

Our study suggests that the SNPs −607 and −137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.