Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum
المؤلفون المشاركون
Montecucco, Fabrizio
Mach, François
Puddu, Alessandra
Sanguineti, Roberta
Nencioni, Alessio
Viviani, Giorgio Luciano
Durante, Arianna
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Advanced glycation end products (AGEs) might play a pathophysiological role in the development of diabetes and its complications.
AGEs negatively affect pancreatic beta-cell function and the expression of transcriptional factors regulating insulin gene.
Glucagon-like peptide-1 (GLP-1), an incretin hormone that regulates glucose homeostasis, might counteract the harmful effects of AGEs on the beta cells in culture.
The aim of this study was to identify the intracellular mechanisms underlying GLP-1-mediated protection from AGE-induced detrimental activities in pancreatic beta cells.
HIT-T15 cells were cultured for 5 days with glycated serum (GS, consisting in a pool of AGEs), in the presence or absence of 10 nmol/L GLP-1.
After evaluation of oxidative stress, we determined the expression and subcellular localization of proteins involved in maintaining redox balance and insulin gene expression, such as nuclear factor erythroid-derived 2 (Nrf2), glutathione reductase, PDX-1, and MafA.
Then, we investigated proinsulin production.
The results showed that GS increased oxidative stress, reduced protein expression of all investigated factors through proteasome activation, and decreased proinsulin content.
Furthermore, GS reduced ability of PDX-1 and MafA to bind DNA.
Coincubation with GLP-1 reversed these GS-mediated detrimental effects.
In conclusion, GLP-1, protecting cells against oxidants, triggers protective intercellular pathways in HIT-T15 cells exposed to GS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Puddu, Alessandra& Sanguineti, Roberta& Durante, Arianna& Nencioni, Alessio& Mach, François& Montecucco, Fabrizio…[et al.]. 2013. Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-462971
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Puddu, Alessandra…[et al.]. Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum. Mediators of Inflammation No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-462971
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Puddu, Alessandra& Sanguineti, Roberta& Durante, Arianna& Nencioni, Alessio& Mach, François& Montecucco, Fabrizio…[et al.]. Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-462971
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-462971
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر