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Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum
Joint Authors
Montecucco, Fabrizio
Mach, François
Puddu, Alessandra
Sanguineti, Roberta
Nencioni, Alessio
Viviani, Giorgio Luciano
Durante, Arianna
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Advanced glycation end products (AGEs) might play a pathophysiological role in the development of diabetes and its complications.
AGEs negatively affect pancreatic beta-cell function and the expression of transcriptional factors regulating insulin gene.
Glucagon-like peptide-1 (GLP-1), an incretin hormone that regulates glucose homeostasis, might counteract the harmful effects of AGEs on the beta cells in culture.
The aim of this study was to identify the intracellular mechanisms underlying GLP-1-mediated protection from AGE-induced detrimental activities in pancreatic beta cells.
HIT-T15 cells were cultured for 5 days with glycated serum (GS, consisting in a pool of AGEs), in the presence or absence of 10 nmol/L GLP-1.
After evaluation of oxidative stress, we determined the expression and subcellular localization of proteins involved in maintaining redox balance and insulin gene expression, such as nuclear factor erythroid-derived 2 (Nrf2), glutathione reductase, PDX-1, and MafA.
Then, we investigated proinsulin production.
The results showed that GS increased oxidative stress, reduced protein expression of all investigated factors through proteasome activation, and decreased proinsulin content.
Furthermore, GS reduced ability of PDX-1 and MafA to bind DNA.
Coincubation with GLP-1 reversed these GS-mediated detrimental effects.
In conclusion, GLP-1, protecting cells against oxidants, triggers protective intercellular pathways in HIT-T15 cells exposed to GS.
American Psychological Association (APA)
Puddu, Alessandra& Sanguineti, Roberta& Durante, Arianna& Nencioni, Alessio& Mach, François& Montecucco, Fabrizio…[et al.]. 2013. Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-462971
Modern Language Association (MLA)
Puddu, Alessandra…[et al.]. Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum. Mediators of Inflammation No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-462971
American Medical Association (AMA)
Puddu, Alessandra& Sanguineti, Roberta& Durante, Arianna& Nencioni, Alessio& Mach, François& Montecucco, Fabrizio…[et al.]. Glucagon-Like Peptide-1 Triggers Protective Pathways in Pancreatic Beta-Cells Exposed to Glycated Serum. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-462971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-462971