A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study
المؤلفون المشاركون
Teo, Jeremy Choon Meng
Zhuang, Yuhang
Ng, Chee Ping
Lin, Alex Wei Haw
المصدر
International Journal of Tissue Engineering
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A bioartificial renal proximal tubule is successfully engineered as a first step towards a bioartificial kidney for improved renal substitution therapy.
To engineer the tubule, a tunable hollow fiber membrane with an exterior skin layer that provides immunoprotection for the cells from extracapillary blood flow and a coarse inner surface that facilitates a hydrogel coating for cell attachment was embedded in a “lab-on-a-chip” model for the small-scale exploratory testing under flow conditions.
Fibrin was coated onto the inner surface of the hollow fiber, and human renal proximal tubule epithelial cells were then seeded.
Using this model, we successfully cultured a confluent monolayer, as ascertained by immunofluorescence staining for ZO-1 tight junctions and other proximal tubule markers, scanning electron microscopy, and FITC-inulin recovery studies.
Furthermore, the inulin studies, combined with the creatinine and glucose transport profiles, suggested that the confluent monolayer exhibits functional transport capabilities.
The novel approaches here may eventually improve current renal substitution technology for renal failure patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ng, Chee Ping& Zhuang, Yuhang& Lin, Alex Wei Haw& Teo, Jeremy Choon Meng. 2012. A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study. International Journal of Tissue Engineering،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463188
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ng, Chee Ping…[et al.]. A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study. International Journal of Tissue Engineering No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-463188
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ng, Chee Ping& Zhuang, Yuhang& Lin, Alex Wei Haw& Teo, Jeremy Choon Meng. A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study. International Journal of Tissue Engineering. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463188
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463188
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر