A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study
Joint Authors
Teo, Jeremy Choon Meng
Zhuang, Yuhang
Ng, Chee Ping
Lin, Alex Wei Haw
Source
International Journal of Tissue Engineering
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A bioartificial renal proximal tubule is successfully engineered as a first step towards a bioartificial kidney for improved renal substitution therapy.
To engineer the tubule, a tunable hollow fiber membrane with an exterior skin layer that provides immunoprotection for the cells from extracapillary blood flow and a coarse inner surface that facilitates a hydrogel coating for cell attachment was embedded in a “lab-on-a-chip” model for the small-scale exploratory testing under flow conditions.
Fibrin was coated onto the inner surface of the hollow fiber, and human renal proximal tubule epithelial cells were then seeded.
Using this model, we successfully cultured a confluent monolayer, as ascertained by immunofluorescence staining for ZO-1 tight junctions and other proximal tubule markers, scanning electron microscopy, and FITC-inulin recovery studies.
Furthermore, the inulin studies, combined with the creatinine and glucose transport profiles, suggested that the confluent monolayer exhibits functional transport capabilities.
The novel approaches here may eventually improve current renal substitution technology for renal failure patients.
American Psychological Association (APA)
Ng, Chee Ping& Zhuang, Yuhang& Lin, Alex Wei Haw& Teo, Jeremy Choon Meng. 2012. A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study. International Journal of Tissue Engineering،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463188
Modern Language Association (MLA)
Ng, Chee Ping…[et al.]. A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study. International Journal of Tissue Engineering No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-463188
American Medical Association (AMA)
Ng, Chee Ping& Zhuang, Yuhang& Lin, Alex Wei Haw& Teo, Jeremy Choon Meng. A Fibrin-Based Tissue-Engineered Renal Proximal Tubule for Bioartificial Kidney Devices : Development, Characterization and In Vitro Transport Study. International Journal of Tissue Engineering. 2012. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463188
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463188