Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice
المؤلفون المشاركون
Lu, Bin
Shao, Jiaqing
Liu, Chunyan
Zhang, Zhen
Wang, Xinyi
Yi, Qiuyan
Wang, Jun
Gan, Zhenhua
Wang, Yingzhijie
Du, Hong
Liu, Yanqing
المصدر
International Journal of Endocrinology
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Recent evidence supported the presence of a local renin-angiotensin system (RAS) in the pancreas, which is implicated in many physiological and pathophysiological processes.
We utilized small interfering RNA (siRNA) to investigate the effects of angiotensin II type 1 receptor (AT1R) knockdown on glucose-stimulated insulin secretion (GSIS) in isolated islets of db/db mice and to explore the potential mechanisms involved.
We found that Ad-siAT1R treatment resulted in a significant decrease both in AT1R mRNA level and in AT1R protein expression level.
With downexpression of AT1R, notable increased insulin secretion and decreased glucagon secretion levels were found by perifusion.
Simultaneously, significant increased protein levels of IRS-1 (by 85%), IRS-2 (by 95%), PI3K(85) (by 112.5%), and p-Akt2 (by 164%) were found by western blot.
And upregulation of both GLUT-2 (by 190%) and GCK (by 121%) was achieved after AT1R inhibition by Ad-siAT1R.
Intraislet AT1R expression level is a crucial physiological regulator of insulin sensitivity of β cell itself and thus affects glucose-induced insulin and glucagon release.
Therefore, the characteristics of AT1R inhibitors could make it a potential novel therapeutics for prevention and treatment of type 2 diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Zhen& Liu, Chunyan& Gan, Zhenhua& Wang, Xinyi& Yi, Qiuyan& Liu, Yanqing…[et al.]. 2013. Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463200
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Zhen…[et al.]. Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice. International Journal of Endocrinology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-463200
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Zhen& Liu, Chunyan& Gan, Zhenhua& Wang, Xinyi& Yi, Qiuyan& Liu, Yanqing…[et al.]. Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463200
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463200
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر