Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice
Joint Authors
Lu, Bin
Shao, Jiaqing
Liu, Chunyan
Zhang, Zhen
Wang, Xinyi
Yi, Qiuyan
Wang, Jun
Gan, Zhenhua
Wang, Yingzhijie
Du, Hong
Liu, Yanqing
Source
International Journal of Endocrinology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Recent evidence supported the presence of a local renin-angiotensin system (RAS) in the pancreas, which is implicated in many physiological and pathophysiological processes.
We utilized small interfering RNA (siRNA) to investigate the effects of angiotensin II type 1 receptor (AT1R) knockdown on glucose-stimulated insulin secretion (GSIS) in isolated islets of db/db mice and to explore the potential mechanisms involved.
We found that Ad-siAT1R treatment resulted in a significant decrease both in AT1R mRNA level and in AT1R protein expression level.
With downexpression of AT1R, notable increased insulin secretion and decreased glucagon secretion levels were found by perifusion.
Simultaneously, significant increased protein levels of IRS-1 (by 85%), IRS-2 (by 95%), PI3K(85) (by 112.5%), and p-Akt2 (by 164%) were found by western blot.
And upregulation of both GLUT-2 (by 190%) and GCK (by 121%) was achieved after AT1R inhibition by Ad-siAT1R.
Intraislet AT1R expression level is a crucial physiological regulator of insulin sensitivity of β cell itself and thus affects glucose-induced insulin and glucagon release.
Therefore, the characteristics of AT1R inhibitors could make it a potential novel therapeutics for prevention and treatment of type 2 diabetes.
American Psychological Association (APA)
Zhang, Zhen& Liu, Chunyan& Gan, Zhenhua& Wang, Xinyi& Yi, Qiuyan& Liu, Yanqing…[et al.]. 2013. Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463200
Modern Language Association (MLA)
Zhang, Zhen…[et al.]. Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice. International Journal of Endocrinology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-463200
American Medical Association (AMA)
Zhang, Zhen& Liu, Chunyan& Gan, Zhenhua& Wang, Xinyi& Yi, Qiuyan& Liu, Yanqing…[et al.]. Improved Glucose-Stimulated Insulin Secretion by Selective Intraislet Inhibition of Angiotensin II Type 1 Receptor Expression in Isolated Islets of dbdb Mice. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-463200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463200