Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth
المؤلفون المشاركون
Suresh, Challa
Chetty, Chellu S.
Chimata, Murali Krishna
المصدر
Biotechnology Research International
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-10-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
The production of extracellular amylase was investigated employing our laboratory isolate, Aspergillus niger sp.
MK 07 and effect of process variables on enzyme production, was studied in a fermentor.
It was found that amylase production was maximum when the fermentor volume was maintained at 70%, rate of agitation at 250 rpm, air supply at 2.5 vvm, inoculum concentration of 10%, and a pH of 5.0.
Highest enzyme production obtained under all optimized conditions was 1734 U/mL with sucrose as carbon substrate and corn steep liquor as nitrogen source.
Enzyme purification studies by ammonium sulphate precipitation and Sephadex G-100 chromatography was evaluated for obtaining purified enzyme.
Thermostability of amylase were evaluated with varying concentrations from 0.2 to 0.5 M concentrations of calcium chloride and the highest activity obtained was 3115 U/mL with 0.3 M calcium chloride at 55°C.
Effect of temperature and pH on the activity of purified enzyme was evaluated and the purified enzyme showed an activity till 75°C and a pH of 6.5.
Application potential of partially purified alpha amylase on desizing of cotton cloth was evaluated with varying enzyme concentrations from 50 to 500 U/mL and the highest desizing activity was found to be at 300 U/mL.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chimata, Murali Krishna& Chetty, Chellu S.& Suresh, Challa. 2011. Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-463484
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chimata, Murali Krishna…[et al.]. Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. Biotechnology Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-463484
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chimata, Murali Krishna& Chetty, Chellu S.& Suresh, Challa. Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-463484
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463484
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر