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Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth
Joint Authors
Suresh, Challa
Chetty, Chellu S.
Chimata, Murali Krishna
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Information Technology and Computer Science
Abstract EN
The production of extracellular amylase was investigated employing our laboratory isolate, Aspergillus niger sp.
MK 07 and effect of process variables on enzyme production, was studied in a fermentor.
It was found that amylase production was maximum when the fermentor volume was maintained at 70%, rate of agitation at 250 rpm, air supply at 2.5 vvm, inoculum concentration of 10%, and a pH of 5.0.
Highest enzyme production obtained under all optimized conditions was 1734 U/mL with sucrose as carbon substrate and corn steep liquor as nitrogen source.
Enzyme purification studies by ammonium sulphate precipitation and Sephadex G-100 chromatography was evaluated for obtaining purified enzyme.
Thermostability of amylase were evaluated with varying concentrations from 0.2 to 0.5 M concentrations of calcium chloride and the highest activity obtained was 3115 U/mL with 0.3 M calcium chloride at 55°C.
Effect of temperature and pH on the activity of purified enzyme was evaluated and the purified enzyme showed an activity till 75°C and a pH of 6.5.
Application potential of partially purified alpha amylase on desizing of cotton cloth was evaluated with varying enzyme concentrations from 50 to 500 U/mL and the highest desizing activity was found to be at 300 U/mL.
American Psychological Association (APA)
Chimata, Murali Krishna& Chetty, Chellu S.& Suresh, Challa. 2011. Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-463484
Modern Language Association (MLA)
Chimata, Murali Krishna…[et al.]. Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. Biotechnology Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-463484
American Medical Association (AMA)
Chimata, Murali Krishna& Chetty, Chellu S.& Suresh, Challa. Fermentative Production and Thermostability Characterization of α Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-463484
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463484