Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines
المؤلفون المشاركون
Madassery, Joseph
Kendre, Gajanan
Cheriyamundath, Sanith
Raghavan, Rahul
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-29
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Tetracycline and glutathione inhibited the protease activities of matrix metalloproteinase-2 and matrix metalloproteinase-9 expressed by mouse fibrosarcoma cells (L929) and Dalton lymphoma cells, respectively.
The inhibitory activity of the tetracycline may be due to its ability to chelate metal ions such as calcium and zinc.
Gelatin-zymography technique was used to demonstrate the inhibitory activity of both tetracycline and glutathione.
The intensity of the bands corresponding to metalloproteinase activity in zymography gel was reduced in the presence of 50–100 μg/mL of tetracycline.
The presence of 10–100 μg/mL of tetracycline in the medium increased the adherence of L929 cancer cells.
These results clearly indicate the antimetastatic property of tetracycline.
Reduced glutathione, a compound which is produced endogenously by the cells to maintain the redox status, was shown to inhibit the matrix metalloproteinase activity (in vitro).
Therefore, it is assumed that decreased glutathione levels in synovial fluids or plasma might increase the activity of MMP.
Reduced glutathione at 100 μg/mL inhibited the metalloproteinase activity in gelatin-zymographic gel.
As both tetracycline and glutathione exhibited an inhibitory effect on matrix metalloproteinase activity, it was of great interest to check their clinical effects on various MMP associated pathological conditions such as cancer metastasis and arthritis.
Here we report that tetracycline and reduced glutathione inhibited the activity of MMP2 completely and activity of MMP9 partly.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kendre, Gajanan& Raghavan, Rahul& Cheriyamundath, Sanith& Madassery, Joseph. 2013. Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines. Journal of Cancer Research،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-463898
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kendre, Gajanan…[et al.]. Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines. Journal of Cancer Research No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-463898
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kendre, Gajanan& Raghavan, Rahul& Cheriyamundath, Sanith& Madassery, Joseph. Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines. Journal of Cancer Research. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-463898
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463898
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر