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Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines
Joint Authors
Madassery, Joseph
Kendre, Gajanan
Cheriyamundath, Sanith
Raghavan, Rahul
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Tetracycline and glutathione inhibited the protease activities of matrix metalloproteinase-2 and matrix metalloproteinase-9 expressed by mouse fibrosarcoma cells (L929) and Dalton lymphoma cells, respectively.
The inhibitory activity of the tetracycline may be due to its ability to chelate metal ions such as calcium and zinc.
Gelatin-zymography technique was used to demonstrate the inhibitory activity of both tetracycline and glutathione.
The intensity of the bands corresponding to metalloproteinase activity in zymography gel was reduced in the presence of 50–100 μg/mL of tetracycline.
The presence of 10–100 μg/mL of tetracycline in the medium increased the adherence of L929 cancer cells.
These results clearly indicate the antimetastatic property of tetracycline.
Reduced glutathione, a compound which is produced endogenously by the cells to maintain the redox status, was shown to inhibit the matrix metalloproteinase activity (in vitro).
Therefore, it is assumed that decreased glutathione levels in synovial fluids or plasma might increase the activity of MMP.
Reduced glutathione at 100 μg/mL inhibited the metalloproteinase activity in gelatin-zymographic gel.
As both tetracycline and glutathione exhibited an inhibitory effect on matrix metalloproteinase activity, it was of great interest to check their clinical effects on various MMP associated pathological conditions such as cancer metastasis and arthritis.
Here we report that tetracycline and reduced glutathione inhibited the activity of MMP2 completely and activity of MMP9 partly.
American Psychological Association (APA)
Kendre, Gajanan& Raghavan, Rahul& Cheriyamundath, Sanith& Madassery, Joseph. 2013. Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines. Journal of Cancer Research،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-463898
Modern Language Association (MLA)
Kendre, Gajanan…[et al.]. Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines. Journal of Cancer Research No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-463898
American Medical Association (AMA)
Kendre, Gajanan& Raghavan, Rahul& Cheriyamundath, Sanith& Madassery, Joseph. Tetracycline and Glutathione Inhibit Matrix Metalloproteinase Activity : An In Vitro Study Using Culture Supernatants of L929 and Dalton Lymphoma Cell Lines. Journal of Cancer Research. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-463898
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463898