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A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress
المؤلفون المشاركون
Mattiello, Luigi
Viretto, Michela
Barale, Cristina
Doronzo, Gabriella
Anfossi, Giovanni
Russo, Isabella
Trovati, Mariella
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A reduction of the nitric oxide (NO) action in vascular smooth muscle cells (VSMC) could play a role in the vascular damage induced by the glycaemic excursions occurring in diabetic patients; in this study, we aimed to clarify whether a short-term incubation of cultured VSMC with high glucose reduces the NO ability to increase cGMP and the cGMP ability to phosphorylate VASP at Ser-239.
We observed that a 180 min incubation of rat VSMC with 25 mmol/L glucose does not impair the NO-induced cGMP increase but reduces VASP phosphorylation in response to both NO and cGMP with a mechanism blunted by antioxidants.
We further demonstrated that high glucose increases radical oxygen species (ROS) production and that this phenomenon is prevented by the PKC inhibitor chelerythrine and the NADPH oxidase inhibitor apocynin.
The following sequence of events is supported by these results: (i) in VSMC high glucose activates PKC; (ii) PKC activates NADPH oxidase; (iii) NADPH oxidase induces oxidative stress; (iv) ROS impair the signalling of cGMP, which is involved in the antiatherogenic actions of NO.
Thus, high glucose, via oxidative stress, can reduce the cardiovascular protection conferred by the NO/cGMP pathway via phosphorylation of the cytoskeleton protein VASP in VSMC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Russo, Isabella& Viretto, Michela& Doronzo, Gabriella& Barale, Cristina& Mattiello, Luigi& Anfossi, Giovanni…[et al.]. 2014. A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-463966
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Russo, Isabella…[et al.]. A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-463966
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Russo, Isabella& Viretto, Michela& Doronzo, Gabriella& Barale, Cristina& Mattiello, Luigi& Anfossi, Giovanni…[et al.]. A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-463966
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463966
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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