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A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress
Joint Authors
Mattiello, Luigi
Viretto, Michela
Barale, Cristina
Doronzo, Gabriella
Anfossi, Giovanni
Russo, Isabella
Trovati, Mariella
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A reduction of the nitric oxide (NO) action in vascular smooth muscle cells (VSMC) could play a role in the vascular damage induced by the glycaemic excursions occurring in diabetic patients; in this study, we aimed to clarify whether a short-term incubation of cultured VSMC with high glucose reduces the NO ability to increase cGMP and the cGMP ability to phosphorylate VASP at Ser-239.
We observed that a 180 min incubation of rat VSMC with 25 mmol/L glucose does not impair the NO-induced cGMP increase but reduces VASP phosphorylation in response to both NO and cGMP with a mechanism blunted by antioxidants.
We further demonstrated that high glucose increases radical oxygen species (ROS) production and that this phenomenon is prevented by the PKC inhibitor chelerythrine and the NADPH oxidase inhibitor apocynin.
The following sequence of events is supported by these results: (i) in VSMC high glucose activates PKC; (ii) PKC activates NADPH oxidase; (iii) NADPH oxidase induces oxidative stress; (iv) ROS impair the signalling of cGMP, which is involved in the antiatherogenic actions of NO.
Thus, high glucose, via oxidative stress, can reduce the cardiovascular protection conferred by the NO/cGMP pathway via phosphorylation of the cytoskeleton protein VASP in VSMC.
American Psychological Association (APA)
Russo, Isabella& Viretto, Michela& Doronzo, Gabriella& Barale, Cristina& Mattiello, Luigi& Anfossi, Giovanni…[et al.]. 2014. A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-463966
Modern Language Association (MLA)
Russo, Isabella…[et al.]. A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-463966
American Medical Association (AMA)
Russo, Isabella& Viretto, Michela& Doronzo, Gabriella& Barale, Cristina& Mattiello, Luigi& Anfossi, Giovanni…[et al.]. A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric OxidecGMP Pathway in Vascular Smooth Muscle Cells : Role of Oxidative Stress. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-463966
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463966