A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus
المؤلفون المشاركون
Wang, He-Xiang
Zhang, Guo-Qing
Ng, Tzi Bun
Sun, Jian
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-29
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Little was known about bioactive compounds from the hallucinogenic mushroom Boletus speciosus.
In the present study, a hemagglutinin (BSH, B.
speciosus hemagglutinin) was isolated from its fruiting bodies and enzymatic properties were also tested.
The chromatographic procedure utilized comprised anion exchange chromatography on Q-Sepharose, cation exchange chromatography on CM-Cellulose, cation exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75.
The hemagglutinin was a homodimer which was estimated to be approximately 31 kDa in size.
The activity of BSH was stable up to 60°C, while there was a precipitous drop in activity when the temperature was elevated to 70°C.
BSH retained 25% hemagglutinating activity when exposed to 100 mM NaOH and 25 mM HCl.
The activity was potently inhibited by 1.25 mM Hg2+ and slightly inhibited by Fe2+, Ca2+, and Pb2+.
None of the sugars tested showed inhibition towards BSH.
Its hemagglutinating activity towards human erythrocytes type A, type B, and type AB was higher than type O.
The hemagglutinin showed antiproliferative activity towards hepatoma Hep G2 cells and mouse lymphocytic leukemia cells (L1210) in vitro, with IC50 of 4.7 μM and 7.0 μM, respectively.
It also exhibited HIV-1 reverse transcriptase inhibitory activity with an IC50 of 7.1 μM.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Jian& Ng, Tzi Bun& Wang, He-Xiang& Zhang, Guo-Qing. 2014. A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-464128
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Jian…[et al.]. A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-464128
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Jian& Ng, Tzi Bun& Wang, He-Xiang& Zhang, Guo-Qing. A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-464128
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464128
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر