A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus
Joint Authors
Wang, He-Xiang
Zhang, Guo-Qing
Ng, Tzi Bun
Sun, Jian
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Little was known about bioactive compounds from the hallucinogenic mushroom Boletus speciosus.
In the present study, a hemagglutinin (BSH, B.
speciosus hemagglutinin) was isolated from its fruiting bodies and enzymatic properties were also tested.
The chromatographic procedure utilized comprised anion exchange chromatography on Q-Sepharose, cation exchange chromatography on CM-Cellulose, cation exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75.
The hemagglutinin was a homodimer which was estimated to be approximately 31 kDa in size.
The activity of BSH was stable up to 60°C, while there was a precipitous drop in activity when the temperature was elevated to 70°C.
BSH retained 25% hemagglutinating activity when exposed to 100 mM NaOH and 25 mM HCl.
The activity was potently inhibited by 1.25 mM Hg2+ and slightly inhibited by Fe2+, Ca2+, and Pb2+.
None of the sugars tested showed inhibition towards BSH.
Its hemagglutinating activity towards human erythrocytes type A, type B, and type AB was higher than type O.
The hemagglutinin showed antiproliferative activity towards hepatoma Hep G2 cells and mouse lymphocytic leukemia cells (L1210) in vitro, with IC50 of 4.7 μM and 7.0 μM, respectively.
It also exhibited HIV-1 reverse transcriptase inhibitory activity with an IC50 of 7.1 μM.
American Psychological Association (APA)
Sun, Jian& Ng, Tzi Bun& Wang, He-Xiang& Zhang, Guo-Qing. 2014. A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-464128
Modern Language Association (MLA)
Sun, Jian…[et al.]. A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-464128
American Medical Association (AMA)
Sun, Jian& Ng, Tzi Bun& Wang, He-Xiang& Zhang, Guo-Qing. A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom Boletus speciosus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-464128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464128