An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus
المؤلفون المشاركون
Romoff, Paulete
Pinho, Marcus Vinícius Terashima de
Toyama, Daniela de Oliveira
Ferreira, Marcelo José Pena
Matioli, Fábio Filippi
Magro, Angelo José
Toyama, Marcos Hikari
Gaeta, Henrique Hessel
Fontes, Marcos Roberto de Mattos
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-18
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
This paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA2) from Crotalus durissus terrificus, which is the main toxin involved in the pharmacological effects of this snake venom.
According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C.
d.
terrificus sPLA2.
Moreover, molecular docking studies showed that quercitrin can establish chemical interactions with some of the crucial amino acid residues involved in the enzymatic activity of the sPLA2, indicating that this flavonoid could also physically impair substrate molecule access to the catalytic site of the toxin.
Additionally, in vitro and in vivo assays showed that the quercitrin strongly diminished the catalytic activity of the protein, altered its Vmax and Km values, and presented a more potent inhibition of essential pharmacological activities in the C.
d.
terrificus sPLA2, such as its myotoxicity and edematogenic effect, in comparison to quercetin.
Thus, we concluded that the rhamnose group found in quercitrin is most likely essential to the antivenom activities of this flavonoid against C.
d.
terrificus sPLA2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Toyama, Daniela de Oliveira& Gaeta, Henrique Hessel& Pinho, Marcus Vinícius Terashima de& Ferreira, Marcelo José Pena& Romoff, Paulete& Matioli, Fábio Filippi…[et al.]. 2014. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-464213
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Toyama, Daniela de Oliveira…[et al.]. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-464213
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Toyama, Daniela de Oliveira& Gaeta, Henrique Hessel& Pinho, Marcus Vinícius Terashima de& Ferreira, Marcelo José Pena& Romoff, Paulete& Matioli, Fábio Filippi…[et al.]. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-464213
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464213
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر