An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus
Joint Authors
Romoff, Paulete
Pinho, Marcus Vinícius Terashima de
Toyama, Daniela de Oliveira
Ferreira, Marcelo José Pena
Matioli, Fábio Filippi
Magro, Angelo José
Toyama, Marcos Hikari
Gaeta, Henrique Hessel
Fontes, Marcos Roberto de Mattos
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This paper shows the results of quercitrin effects on the structure and biological activity of secretory phospholipase (sPLA2) from Crotalus durissus terrificus, which is the main toxin involved in the pharmacological effects of this snake venom.
According to our mass spectrometry and circular dichroism results, quercetin was able to promote a chemical modification of some amino acid residues and modify the secondary structure of C.
d.
terrificus sPLA2.
Moreover, molecular docking studies showed that quercitrin can establish chemical interactions with some of the crucial amino acid residues involved in the enzymatic activity of the sPLA2, indicating that this flavonoid could also physically impair substrate molecule access to the catalytic site of the toxin.
Additionally, in vitro and in vivo assays showed that the quercitrin strongly diminished the catalytic activity of the protein, altered its Vmax and Km values, and presented a more potent inhibition of essential pharmacological activities in the C.
d.
terrificus sPLA2, such as its myotoxicity and edematogenic effect, in comparison to quercetin.
Thus, we concluded that the rhamnose group found in quercitrin is most likely essential to the antivenom activities of this flavonoid against C.
d.
terrificus sPLA2.
American Psychological Association (APA)
Toyama, Daniela de Oliveira& Gaeta, Henrique Hessel& Pinho, Marcus Vinícius Terashima de& Ferreira, Marcelo José Pena& Romoff, Paulete& Matioli, Fábio Filippi…[et al.]. 2014. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-464213
Modern Language Association (MLA)
Toyama, Daniela de Oliveira…[et al.]. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-464213
American Medical Association (AMA)
Toyama, Daniela de Oliveira& Gaeta, Henrique Hessel& Pinho, Marcus Vinícius Terashima de& Ferreira, Marcelo José Pena& Romoff, Paulete& Matioli, Fábio Filippi…[et al.]. An Evaluation of 3-Rhamnosylquercetin, a Glycosylated Form of Quercetin, against the Myotoxic and Edematogenic Effects of sPLA2 from Crotalus durissus terrificus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-464213
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464213