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Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium
المؤلفون المشاركون
Martínez, Nhora
Gallego, Carolina
Middleton, Andrew M.
Romero, Stefany
Iregui, Carlos
المصدر
Veterinary Medicine International
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The nasal septa of fetal rabbits at 26 days of gestation were harvested by cesarean section of the does while under anesthesia and then exposed to Bordetella bronchiseptica or its lipopolysaccharide (LPS) for periods of 2 and 4 hours.
A total of 240 explants were used.
The tissues were examined using the Hematoxylin & Eosin technique.
Then, semithin sections (0.5 μm) were stained with toluidine blue and examined with indirect immunoperoxidase (IPI) and lectin histochemistry.
The most frequent and statistically significant findings were as follows: (1) cell death and increased goblet cell activity when exposed to bacteria and (2) cell death, cytoplasmic vacuolation and infiltration of polymorphonuclear leukocytes when exposed to LPS.
The lesions induced by the bacterium were more severe than with LPS alone, except for the cytoplasmic vacuolation in epithelial cells.
IPI stained the ciliated border of the epithelium with the bacterium more intensely, while LPS lectin histochemistry preferentially labeled the cytoplasm of goblet cell.
These data indicate that B.
bronchiseptica and its LPS may have an affinity for specific glycoproteins that would act as adhesion receptors in both locations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gallego, Carolina& Middleton, Andrew M.& Martínez, Nhora& Romero, Stefany& Iregui, Carlos. 2013. Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Veterinary Medicine International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-464612
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gallego, Carolina…[et al.]. Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Veterinary Medicine International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-464612
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gallego, Carolina& Middleton, Andrew M.& Martínez, Nhora& Romero, Stefany& Iregui, Carlos. Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Veterinary Medicine International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-464612
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464612
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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