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Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium
Joint Authors
Martínez, Nhora
Gallego, Carolina
Middleton, Andrew M.
Romero, Stefany
Iregui, Carlos
Source
Veterinary Medicine International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The nasal septa of fetal rabbits at 26 days of gestation were harvested by cesarean section of the does while under anesthesia and then exposed to Bordetella bronchiseptica or its lipopolysaccharide (LPS) for periods of 2 and 4 hours.
A total of 240 explants were used.
The tissues were examined using the Hematoxylin & Eosin technique.
Then, semithin sections (0.5 μm) were stained with toluidine blue and examined with indirect immunoperoxidase (IPI) and lectin histochemistry.
The most frequent and statistically significant findings were as follows: (1) cell death and increased goblet cell activity when exposed to bacteria and (2) cell death, cytoplasmic vacuolation and infiltration of polymorphonuclear leukocytes when exposed to LPS.
The lesions induced by the bacterium were more severe than with LPS alone, except for the cytoplasmic vacuolation in epithelial cells.
IPI stained the ciliated border of the epithelium with the bacterium more intensely, while LPS lectin histochemistry preferentially labeled the cytoplasm of goblet cell.
These data indicate that B.
bronchiseptica and its LPS may have an affinity for specific glycoproteins that would act as adhesion receptors in both locations.
American Psychological Association (APA)
Gallego, Carolina& Middleton, Andrew M.& Martínez, Nhora& Romero, Stefany& Iregui, Carlos. 2013. Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Veterinary Medicine International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-464612
Modern Language Association (MLA)
Gallego, Carolina…[et al.]. Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Veterinary Medicine International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-464612
American Medical Association (AMA)
Gallego, Carolina& Middleton, Andrew M.& Martínez, Nhora& Romero, Stefany& Iregui, Carlos. Interaction of Bordetella bronchiseptica and Its Lipopolysaccharide with In Vitro Culture of Respiratory Nasal Epithelium. Veterinary Medicine International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-464612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464612