![](/images/graphics-bg.png)
The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis
المؤلفون المشاركون
Oduaran, Erica
Eram, Mohammad S.
Ma, Kesen
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-29
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The hyperthermophilic archaeon Thermococcus guaymasensis produces ethanol as a metabolic end product, and an alcohol dehydrogenase (ADH) catalyzing the reduction of acetaldehyde to ethanol has been purified and characterized.
However, the enzyme catalyzing the formation of acetaldehyde has not been identified.
In this study an enzyme catalyzing the production of acetaldehyde from pyruvate was purified and characterized from T.
guaymasensis under strictly anaerobic conditions.
The enzyme had both pyruvate decarboxylase (PDC) and pyruvate ferredoxin oxidoreductase (POR) activities.
It was oxygen sensitive, and the optimal temperatures were 85°C and >95°C for the PDC and POR activities, respectively.
The purified enzyme had activities of 3.8±0.22 U mg−1 and 20.2±1.8 U mg−1, with optimal pH-values of 9.5 and 8.4 for each activity, respectively.
Coenzyme A was essential for both activities, although it did not serve as a substrate for the former.
Enzyme kinetic parameters were determined separately for each activity.
The purified enzyme was a heterotetramer.
The sequences of the genes encoding the subunits of the bifunctional PDC/POR were determined.
It is predicted that all hyperthermophilic β-keto acids ferredoxin oxidoreductases are bifunctional, catalyzing the activities of nonoxidative and oxidative decarboxylation of the corresponding β-keto acids.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Eram, Mohammad S.& Oduaran, Erica& Ma, Kesen. 2014. The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis. Archaea،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-464829
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Eram, Mohammad S.…[et al.]. The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis. Archaea No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-464829
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Eram, Mohammad S.& Oduaran, Erica& Ma, Kesen. The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis. Archaea. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-464829
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464829
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)