The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis
Joint Authors
Oduaran, Erica
Eram, Mohammad S.
Ma, Kesen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The hyperthermophilic archaeon Thermococcus guaymasensis produces ethanol as a metabolic end product, and an alcohol dehydrogenase (ADH) catalyzing the reduction of acetaldehyde to ethanol has been purified and characterized.
However, the enzyme catalyzing the formation of acetaldehyde has not been identified.
In this study an enzyme catalyzing the production of acetaldehyde from pyruvate was purified and characterized from T.
guaymasensis under strictly anaerobic conditions.
The enzyme had both pyruvate decarboxylase (PDC) and pyruvate ferredoxin oxidoreductase (POR) activities.
It was oxygen sensitive, and the optimal temperatures were 85°C and >95°C for the PDC and POR activities, respectively.
The purified enzyme had activities of 3.8±0.22 U mg−1 and 20.2±1.8 U mg−1, with optimal pH-values of 9.5 and 8.4 for each activity, respectively.
Coenzyme A was essential for both activities, although it did not serve as a substrate for the former.
Enzyme kinetic parameters were determined separately for each activity.
The purified enzyme was a heterotetramer.
The sequences of the genes encoding the subunits of the bifunctional PDC/POR were determined.
It is predicted that all hyperthermophilic β-keto acids ferredoxin oxidoreductases are bifunctional, catalyzing the activities of nonoxidative and oxidative decarboxylation of the corresponding β-keto acids.
American Psychological Association (APA)
Eram, Mohammad S.& Oduaran, Erica& Ma, Kesen. 2014. The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis. Archaea،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-464829
Modern Language Association (MLA)
Eram, Mohammad S.…[et al.]. The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis. Archaea No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-464829
American Medical Association (AMA)
Eram, Mohammad S.& Oduaran, Erica& Ma, Kesen. The Bifunctional Pyruvate DecarboxylasePyruvate Ferredoxin Oxidoreductase from Thermococcus guaymasensis. Archaea. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-464829
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464829