Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection
المؤلفون المشاركون
Geletneky, Karsten
Schlehofer, Joerg R.
Hartkopf, Andreas D.
Krempien, Robert
Rommelaere, Jean
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-03-07
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Purpose.
To elucidate the influence of ionizing radiation (IR) on the oncolytic activity of Parvovirus H-1 (H-1PV) in human high-grade glioma cells.
Methods.
Short term cultures of human high-grade gliomas were irradiated at different doses and infected with H-1PV.
Cell viability was assessed by determining relative numbers of surviving cells.
Replication of H-1PV was measured by RT-PCR of viral RNA, fluorescence-activated cell sorter (FACS) analysis and the synthesis of infectious virus particles.
To identify a possible mechanism for radiation induced change in the oncolytic activity of H-1PV we performed cell cycle analyses.
Results.
Previous irradiation rendered glioma cells fully permissive to H-1PV infection.
Irradiation 24 hours prior to H-1PV infection led to increased cell killing most notably in radioresistant glioma cells.
Intracellular levels of NS-1, the main effector of H-1PV induced cytotoxicity, were elevated after irradiation.
S-phase levels were increased one day after irradiation improving S-phase dependent viral replication and cytotoxicity.
Conclusion.
This study demonstrates intact susceptibility of previously irradiated glioma-cells for H-1PV induced oncolysis.
The combination of ionizing radiation followed by H-1PV infection increased viral cytotoxicity, especially in radioresistant gliomas.
These findings support the ongoing development of a clinical trial of H-1PV in patients with recurrent glioblastomas.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Geletneky, Karsten& Hartkopf, Andreas D.& Krempien, Robert& Rommelaere, Jean& Schlehofer, Joerg R.. 2010. Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-464937
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Geletneky, Karsten…[et al.]. Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-464937
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Geletneky, Karsten& Hartkopf, Andreas D.& Krempien, Robert& Rommelaere, Jean& Schlehofer, Joerg R.. Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-464937
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464937
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر