Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection
Joint Authors
Geletneky, Karsten
Schlehofer, Joerg R.
Hartkopf, Andreas D.
Krempien, Robert
Rommelaere, Jean
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
To elucidate the influence of ionizing radiation (IR) on the oncolytic activity of Parvovirus H-1 (H-1PV) in human high-grade glioma cells.
Methods.
Short term cultures of human high-grade gliomas were irradiated at different doses and infected with H-1PV.
Cell viability was assessed by determining relative numbers of surviving cells.
Replication of H-1PV was measured by RT-PCR of viral RNA, fluorescence-activated cell sorter (FACS) analysis and the synthesis of infectious virus particles.
To identify a possible mechanism for radiation induced change in the oncolytic activity of H-1PV we performed cell cycle analyses.
Results.
Previous irradiation rendered glioma cells fully permissive to H-1PV infection.
Irradiation 24 hours prior to H-1PV infection led to increased cell killing most notably in radioresistant glioma cells.
Intracellular levels of NS-1, the main effector of H-1PV induced cytotoxicity, were elevated after irradiation.
S-phase levels were increased one day after irradiation improving S-phase dependent viral replication and cytotoxicity.
Conclusion.
This study demonstrates intact susceptibility of previously irradiated glioma-cells for H-1PV induced oncolysis.
The combination of ionizing radiation followed by H-1PV infection increased viral cytotoxicity, especially in radioresistant gliomas.
These findings support the ongoing development of a clinical trial of H-1PV in patients with recurrent glioblastomas.
American Psychological Association (APA)
Geletneky, Karsten& Hartkopf, Andreas D.& Krempien, Robert& Rommelaere, Jean& Schlehofer, Joerg R.. 2010. Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-464937
Modern Language Association (MLA)
Geletneky, Karsten…[et al.]. Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-464937
American Medical Association (AMA)
Geletneky, Karsten& Hartkopf, Andreas D.& Krempien, Robert& Rommelaere, Jean& Schlehofer, Joerg R.. Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-464937
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464937