Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells
المؤلفون المشاركون
Chung, Eunna
Rylander, Marissa Nichole
Sampson, Alana Cherrell
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-11
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Stress conditioning (e.g., thermal, shear, and tensile stress) of bone cells has been shown to enhance healing.
However, prior studies have not investigated whether combined stress could synergistically promote bone regeneration.
This study explored the impact of combined thermal and tensile stress on the induction of heat shock proteins (HSPs) and bone-related proteins by a murine preosteoblast cell line (MC3T3-E1).
Cells were exposed to thermal stress using a water bath (44°C for 4 or 8 minutes) with postheating incubation (37°C for 4 hours) followed by exposure to cyclic strain (equibiaxial 3%, 0.2 Hz, cycle of 10-second tensile stress followed by 10-second rest).
Combined thermal stress and tensile stress induced mRNA expression of HSP27 (1.41 relative fold induction (RFI) compared to sham-treated control), HSP70 (5.55 RFI), and osteopontin (1.44 RFI) but suppressed matrix metalloproteinase-9 (0.6 RFI) compared to the control.
Combined thermal and tensile stress increased vascular endothelial growth factor (VEGF) secretion into the culture supernatant (1.54-fold increase compared to the control).
Therefore, combined thermal and mechanical stress preconditioning can enhance HSP induction and influence protein expression important for bone tissue healing.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chung, Eunna& Sampson, Alana Cherrell& Rylander, Marissa Nichole. 2014. Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-465291
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chung, Eunna…[et al.]. Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells. BioMed Research International No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-465291
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chung, Eunna& Sampson, Alana Cherrell& Rylander, Marissa Nichole. Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-465291
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465291
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر