Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells
Joint Authors
Chung, Eunna
Rylander, Marissa Nichole
Sampson, Alana Cherrell
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-11
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Stress conditioning (e.g., thermal, shear, and tensile stress) of bone cells has been shown to enhance healing.
However, prior studies have not investigated whether combined stress could synergistically promote bone regeneration.
This study explored the impact of combined thermal and tensile stress on the induction of heat shock proteins (HSPs) and bone-related proteins by a murine preosteoblast cell line (MC3T3-E1).
Cells were exposed to thermal stress using a water bath (44°C for 4 or 8 minutes) with postheating incubation (37°C for 4 hours) followed by exposure to cyclic strain (equibiaxial 3%, 0.2 Hz, cycle of 10-second tensile stress followed by 10-second rest).
Combined thermal stress and tensile stress induced mRNA expression of HSP27 (1.41 relative fold induction (RFI) compared to sham-treated control), HSP70 (5.55 RFI), and osteopontin (1.44 RFI) but suppressed matrix metalloproteinase-9 (0.6 RFI) compared to the control.
Combined thermal and tensile stress increased vascular endothelial growth factor (VEGF) secretion into the culture supernatant (1.54-fold increase compared to the control).
Therefore, combined thermal and mechanical stress preconditioning can enhance HSP induction and influence protein expression important for bone tissue healing.
American Psychological Association (APA)
Chung, Eunna& Sampson, Alana Cherrell& Rylander, Marissa Nichole. 2014. Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-465291
Modern Language Association (MLA)
Chung, Eunna…[et al.]. Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells. BioMed Research International No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-465291
American Medical Association (AMA)
Chung, Eunna& Sampson, Alana Cherrell& Rylander, Marissa Nichole. Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-465291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465291