RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts
المؤلفون المشاركون
Sun, Yuncheng
Li, Tiejun
Jing, Jiaona
Li, Ping
Guan, Huaijin
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Purpose.
This study was to determine the effect of CTGF-small interfering RNA (siRNA) on TGF-β2-induced proliferation in human Tenon capsule fibroblasts (HTFs).
Methods.
HTFs were transfected with four of CTGF-siRNAs separately for screening of gene silencing efficacy that was determined by transcript level measured by quantitative real-time PCR (qRT-PCR).
Recombinant TGF-β2 was added into the culture to stimulate the proliferation of HTFs.
The gene silencing efficacy of the siRNAs was evaluated by qRT-PCR and immunofluorescence of CTGF transcript and protein levels.
The viability of HTFs was determined by cell counting kit-8 (CCK-8).
FCM was used to assess cell cycle after CTGF-siRNA transfection.
Results.
The expression of CTGF and proliferation of HTFs were increased significantly by TGF-β2 stimulation.
The transfection of CTGF-siRNA abolished the upregulation of CTGF and cell proliferation induced by TGF-β2.
The analysis of cell cycle indicated that CTGF-siRNA treatment stimulated cells from S phase to G0/G1 phase in comparison with the inverse physiologic function of TGF-β2.
Conclusion.
CTGF targeting siRNA could effectively suppress the expression of CTGF and attenuate the proliferation of HTFs.
The siRNA approach may provide a therapeutic option for eliminating filtration bleb scarring after glaucoma filtration surgery (GFS).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jing, Jiaona& Li, Ping& Li, Tiejun& Sun, Yuncheng& Guan, Huaijin. 2013. RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts. Journal of Ophthalmology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-465342
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jing, Jiaona…[et al.]. RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts. Journal of Ophthalmology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-465342
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jing, Jiaona& Li, Ping& Li, Tiejun& Sun, Yuncheng& Guan, Huaijin. RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts. Journal of Ophthalmology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-465342
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465342
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر