RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts
Joint Authors
Sun, Yuncheng
Li, Tiejun
Jing, Jiaona
Li, Ping
Guan, Huaijin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
This study was to determine the effect of CTGF-small interfering RNA (siRNA) on TGF-β2-induced proliferation in human Tenon capsule fibroblasts (HTFs).
Methods.
HTFs were transfected with four of CTGF-siRNAs separately for screening of gene silencing efficacy that was determined by transcript level measured by quantitative real-time PCR (qRT-PCR).
Recombinant TGF-β2 was added into the culture to stimulate the proliferation of HTFs.
The gene silencing efficacy of the siRNAs was evaluated by qRT-PCR and immunofluorescence of CTGF transcript and protein levels.
The viability of HTFs was determined by cell counting kit-8 (CCK-8).
FCM was used to assess cell cycle after CTGF-siRNA transfection.
Results.
The expression of CTGF and proliferation of HTFs were increased significantly by TGF-β2 stimulation.
The transfection of CTGF-siRNA abolished the upregulation of CTGF and cell proliferation induced by TGF-β2.
The analysis of cell cycle indicated that CTGF-siRNA treatment stimulated cells from S phase to G0/G1 phase in comparison with the inverse physiologic function of TGF-β2.
Conclusion.
CTGF targeting siRNA could effectively suppress the expression of CTGF and attenuate the proliferation of HTFs.
The siRNA approach may provide a therapeutic option for eliminating filtration bleb scarring after glaucoma filtration surgery (GFS).
American Psychological Association (APA)
Jing, Jiaona& Li, Ping& Li, Tiejun& Sun, Yuncheng& Guan, Huaijin. 2013. RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts. Journal of Ophthalmology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-465342
Modern Language Association (MLA)
Jing, Jiaona…[et al.]. RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts. Journal of Ophthalmology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-465342
American Medical Association (AMA)
Jing, Jiaona& Li, Ping& Li, Tiejun& Sun, Yuncheng& Guan, Huaijin. RNA Interference Targeting Connective Tissue Growth Factor Inhibits the Transforming Growth Factor-β2 Induced Proliferation in Human Tenon Capsule Fibroblasts. Journal of Ophthalmology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-465342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465342