![](/images/graphics-bg.png)
Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell
المؤلفون المشاركون
Yang, Nian-yun
Wang, Ming-yan
Yuan, Xiao-lin
Jiang, Li-hua
Zou, Yi-jie
Lu, Da-xiang
Zhao, Feng-ming
Chen, Jian-ping
Jiang, Ze-qun
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-11
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Neural stem cells (NSCs) are self-regenerating cells, but their regenerative capacity is limited.
The present study was conducted to investigate the effect of β-sitosterol-D-glucoside (BSSG) on the proliferation of hippocampal NSCs and to determine the corresponding molecular mechanism.
Results of CCK-8 assay showed that BSSG significantly increased NSC proliferation and the effectiveness of BSSG was similar to that of basic fibroblast growth factor and epidermal growth factor.
mRNA expression profiling showed that 960 genes were differentially expressed after NSCs were treated with BSSG.
Among the 960 genes, IGF1 is considered as a key regulatory gene that functionally promotes NSC proliferation.
MicroRNA (miRNA) expression profiling indicated that 30 and 84 miRNAs were upregulated and downregulated, respectively.
miRNA-mRNA relevance analysis revealed that numerous mRNAs including IGF1 mRNA were negatively regulated by miRNAs with decreased expression, thereby increasing the corresponding mRNA expression.
The increased expression of IGF1 protein was validated by ELISA.
Picropodophyllin (PPP, an inhibitor of IGF-1R) inhibition test confirmed that the proliferation-enhancing effect depended on IGF1.
This study provided information about BSSG as an efficient and inexpensive growth factor alternative, of which the effect is closely involved in IGF1.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Li-hua& Yang, Nian-yun& Yuan, Xiao-lin& Zou, Yi-jie& Jiang, Ze-qun& Zhao, Feng-ming…[et al.]. 2013. Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-465792
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Li-hua…[et al.]. Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-465792
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Li-hua& Yang, Nian-yun& Yuan, Xiao-lin& Zou, Yi-jie& Jiang, Ze-qun& Zhao, Feng-ming…[et al.]. Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-465792
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465792
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)