Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell
Joint Authors
Yang, Nian-yun
Wang, Ming-yan
Yuan, Xiao-lin
Jiang, Li-hua
Zou, Yi-jie
Lu, Da-xiang
Zhao, Feng-ming
Chen, Jian-ping
Jiang, Ze-qun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Neural stem cells (NSCs) are self-regenerating cells, but their regenerative capacity is limited.
The present study was conducted to investigate the effect of β-sitosterol-D-glucoside (BSSG) on the proliferation of hippocampal NSCs and to determine the corresponding molecular mechanism.
Results of CCK-8 assay showed that BSSG significantly increased NSC proliferation and the effectiveness of BSSG was similar to that of basic fibroblast growth factor and epidermal growth factor.
mRNA expression profiling showed that 960 genes were differentially expressed after NSCs were treated with BSSG.
Among the 960 genes, IGF1 is considered as a key regulatory gene that functionally promotes NSC proliferation.
MicroRNA (miRNA) expression profiling indicated that 30 and 84 miRNAs were upregulated and downregulated, respectively.
miRNA-mRNA relevance analysis revealed that numerous mRNAs including IGF1 mRNA were negatively regulated by miRNAs with decreased expression, thereby increasing the corresponding mRNA expression.
The increased expression of IGF1 protein was validated by ELISA.
Picropodophyllin (PPP, an inhibitor of IGF-1R) inhibition test confirmed that the proliferation-enhancing effect depended on IGF1.
This study provided information about BSSG as an efficient and inexpensive growth factor alternative, of which the effect is closely involved in IGF1.
American Psychological Association (APA)
Jiang, Li-hua& Yang, Nian-yun& Yuan, Xiao-lin& Zou, Yi-jie& Jiang, Ze-qun& Zhao, Feng-ming…[et al.]. 2013. Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-465792
Modern Language Association (MLA)
Jiang, Li-hua…[et al.]. Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-465792
American Medical Association (AMA)
Jiang, Li-hua& Yang, Nian-yun& Yuan, Xiao-lin& Zou, Yi-jie& Jiang, Ze-qun& Zhao, Feng-ming…[et al.]. Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-465792
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465792