![](/images/graphics-bg.png)
Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone
المؤلفون المشاركون
Marzi, Ingo
Barker, John
Wilhelm, Kerstin
Frank, Johannes
Henrich, Dirk
Seebach, Caroline
Warzecha, Joerg
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-17
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Introduction.
Cancellous bone is frequently used for filling bone defects in a clinical setting.
It provides favourable conditions for regenerative cells such as MSC and early EPC.
The combination of MSC and EPC results in superior bone healing in experimental bone healing models.
Materials and Methods.
We investigated the influence of osteogenic culture conditions on the endothelial properties of early EPC and the osteogenic properties of MSC when cocultured on cancellous bone.
Additionally, cell adhesion, metabolic activity, and differentiation were assessed 2, 6, and 10 days after seeding.
Results.
The number of adhering EPC and MSC decreased over time; however the cells remained metabolically active over the 10-day measurement period.
In spite of a decline of lineage specific markers, cells maintained their differentiation to a reduced level.
Osteogenic stimulation of EPC caused a decline but not abolishment of endothelial characteristics and did not induce osteogenic gene expression.
Osteogenic stimulation of MSC significantly increased their metabolic activity whereas collagen-1α and alkaline phosphatase gene expressions declined.
When cocultured with EPC, MSC’s collagen-1α gene expression increased significantly.
Conclusion.
EPC and MSC can be cocultured in vitro on cancellous bone under osteogenic conditions, and coculturing EPC with MSC stabilizes the latter’s collagen-1α gene expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Henrich, Dirk& Wilhelm, Kerstin& Warzecha, Joerg& Frank, Johannes& Barker, John& Marzi, Ingo…[et al.]. 2013. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-466161
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Henrich, Dirk…[et al.]. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-466161
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Henrich, Dirk& Wilhelm, Kerstin& Warzecha, Joerg& Frank, Johannes& Barker, John& Marzi, Ingo…[et al.]. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-466161
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-466161
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)