Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone
Joint Authors
Marzi, Ingo
Barker, John
Wilhelm, Kerstin
Frank, Johannes
Henrich, Dirk
Seebach, Caroline
Warzecha, Joerg
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Introduction.
Cancellous bone is frequently used for filling bone defects in a clinical setting.
It provides favourable conditions for regenerative cells such as MSC and early EPC.
The combination of MSC and EPC results in superior bone healing in experimental bone healing models.
Materials and Methods.
We investigated the influence of osteogenic culture conditions on the endothelial properties of early EPC and the osteogenic properties of MSC when cocultured on cancellous bone.
Additionally, cell adhesion, metabolic activity, and differentiation were assessed 2, 6, and 10 days after seeding.
Results.
The number of adhering EPC and MSC decreased over time; however the cells remained metabolically active over the 10-day measurement period.
In spite of a decline of lineage specific markers, cells maintained their differentiation to a reduced level.
Osteogenic stimulation of EPC caused a decline but not abolishment of endothelial characteristics and did not induce osteogenic gene expression.
Osteogenic stimulation of MSC significantly increased their metabolic activity whereas collagen-1α and alkaline phosphatase gene expressions declined.
When cocultured with EPC, MSC’s collagen-1α gene expression increased significantly.
Conclusion.
EPC and MSC can be cocultured in vitro on cancellous bone under osteogenic conditions, and coculturing EPC with MSC stabilizes the latter’s collagen-1α gene expression.
American Psychological Association (APA)
Henrich, Dirk& Wilhelm, Kerstin& Warzecha, Joerg& Frank, Johannes& Barker, John& Marzi, Ingo…[et al.]. 2013. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-466161
Modern Language Association (MLA)
Henrich, Dirk…[et al.]. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-466161
American Medical Association (AMA)
Henrich, Dirk& Wilhelm, Kerstin& Warzecha, Joerg& Frank, Johannes& Barker, John& Marzi, Ingo…[et al.]. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-466161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-466161