Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone

Joint Authors

Marzi, Ingo
Barker, John
Wilhelm, Kerstin
Frank, Johannes
Henrich, Dirk
Seebach, Caroline
Warzecha, Joerg

Source

Mediators of Inflammation

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-02-17

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Diseases

Abstract EN

Introduction.

Cancellous bone is frequently used for filling bone defects in a clinical setting.

It provides favourable conditions for regenerative cells such as MSC and early EPC.

The combination of MSC and EPC results in superior bone healing in experimental bone healing models.

Materials and Methods.

We investigated the influence of osteogenic culture conditions on the endothelial properties of early EPC and the osteogenic properties of MSC when cocultured on cancellous bone.

Additionally, cell adhesion, metabolic activity, and differentiation were assessed 2, 6, and 10 days after seeding.

Results.

The number of adhering EPC and MSC decreased over time; however the cells remained metabolically active over the 10-day measurement period.

In spite of a decline of lineage specific markers, cells maintained their differentiation to a reduced level.

Osteogenic stimulation of EPC caused a decline but not abolishment of endothelial characteristics and did not induce osteogenic gene expression.

Osteogenic stimulation of MSC significantly increased their metabolic activity whereas collagen-1α and alkaline phosphatase gene expressions declined.

When cocultured with EPC, MSC’s collagen-1α gene expression increased significantly.

Conclusion.

EPC and MSC can be cocultured in vitro on cancellous bone under osteogenic conditions, and coculturing EPC with MSC stabilizes the latter’s collagen-1α gene expression.

American Psychological Association (APA)

Henrich, Dirk& Wilhelm, Kerstin& Warzecha, Joerg& Frank, Johannes& Barker, John& Marzi, Ingo…[et al.]. 2013. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-466161

Modern Language Association (MLA)

Henrich, Dirk…[et al.]. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-466161

American Medical Association (AMA)

Henrich, Dirk& Wilhelm, Kerstin& Warzecha, Joerg& Frank, Johannes& Barker, John& Marzi, Ingo…[et al.]. Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-466161

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-466161